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EVENT DETAILS |
Class in four sessions:
December 4, 5, 6 & 13
6:00 PM - 10:00 PM
CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes & increasing its accessibility. Want to learn how to do hands-on genome editing? This is an intensive laboratory class with limited space. Class size is limited to ten students.
In this class you will:
Learn how to culture & work withSaccharomyces cerevisciae(brewers yeast)
Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion
Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome
Use the constructs you produce to transform yeastand confirm the results by DNA sequence analysis
Prerequisites:
You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes & PCR (Genspace Biotech Crash Course, Biohacker Boot Camp or equivalent lab experience)
Will Shindel is the Laboratory Manager at Genspace & also provides scientific mentoring to our members. He studied Biology at Arcadia University, where he investigated the role of insulator elements in genome organization. After graduating, he spent several years working in the pharmaceutical industry. It was during this time that he discovered DIY biology & first came to Genspace. In addition to being Laboratory Manager, Will also teaches the course Genome Editing with CRISPR Cas-9 & is working on creating a spicy tomato by engineering in the capsaicin pathway genes.
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